Anti-PCSK9 Antibody Picoband™

Product Name

Anti-PCSK9 Antibody Picoband™

View all Proprotein Convertase 9/PCSK9 Antibodies

SKU/Catalog Number

A00085-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCSK9 Antibody Picoband™ catalog # A00085-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCSK9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00085-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PCSK9 recombinant protein (Position: S153-Q344).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00085-1 is reactive to Pcsk9 in Human

Applications

A00085-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

66 kDa, 80 kDa (precursor)

Calculated molecular weight

74.823kDa

Background of Proprotein Convertase 9/PCSK9

Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, PCSK9 was mapped to chromosome 1p32. This gene is a crucial player in the regulation of plasma cholesterol homeostasis. It may prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. PCSK9 can induce ubiquitination of LDLR leading to its subsequent degradation. This gene is involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA(Cap), 1-5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PCSK9 using anti-PCSK9 antibody (A00085-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # A00085-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa, 80 kDa (precursor). The expected band size for PCSK9 is at 72 kDa.

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Figure 2. IHC analysis of PCSK9 using anti PCSK9 antibody (A00085-1).
PCSK9 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCSK9 Antibody (A00085-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. Sandwich ELISA - Recombinant human PCSK9 protein standard curve.
Use in combination with reagents from Human PCSK9 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1147).

Gene/Protein Information For Pcsk9 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9 Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9

For more info on Pcsk9, check out the Pcsk9 Infographic

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In this infographic, you will see the following information for Pcsk9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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